1.
Indian J Exp Biol
;
1988 Mar; 26(3): 195-200
Article
in English
| IMSEAR
| ID: sea-61469
2.
J Biosci
;
1981 Sept; 3(3): 293-302
Article
in English
| IMSEAR
| ID: sea-160160
ABSTRACT
A rapid method for the preparation of sarcolema from frog skeletal muscle has been described. The purified cell segments were transparent and devoid of contractile material. The Na+, Κ+ -ATPase and 5'-nucleotidase activities in sarcolemma purified by this method were comparable to those reported for sarcolemmal preparations purified by density gradient centrifugation. The preparation also possessed acid phosphatase, alkaline phosphatase and Κ+-activated, ouabain-sensitive p-nitrophenyl phosphatase activities. The cholesterol to phospholipid ratio of the sarcolemma was 0.33, indicating its high purity; further, the preparation was free from mitochondria and contractile proteins.